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Please use this identifier to cite or link to this item: http://ir.hwai.edu.tw:8080/ir/handle/310996100Q/48

Title: 免疫球蛋白E現場即時檢測電化學生物感測器之研製
Authors: 殷立德
Keywords: 硝化纖維素
延伸式閘極場效電晶體(EGFET)
SnO2/ITO
spin-coated 技術
尿素
尿素酶
nitrocellulose
extended-gate field effect transistors (EGFET)
spin-coated technology
urease
urea
Date: 2007
Issue Date: 2009-05-08 14:57:19 (UTC+8)
Abstract: 硝化纖維素薄膜是一種高品質的薄膜,應用於西方墨點法、南方墨點法、北方墨點法的轉
漬、快速免疫色層分析法和蛋白質微陣列,主要用來固定蛋白質和核酸,在本篇研究,我
們首先提出硝化纖維素在延伸式閘極場效電晶體(EGFET)的修飾。硝化纖維素薄膜利用
spin-coated 技術直接附著於SnO2/ITO 閘極上,在製作硝化纖維素薄膜的過程中我們發現硝
化纖維素和純水的濃度、spin coater 的轉速與此薄膜是否能夠做最有效的作用有關,因此我
們利用pH sensitive 實驗來得知元件的特性及感測度。結果發現用3%純水、9%硝化纖維素
及3000 rpm 的轉速來固定的EGFET 最具備有好的感測度,感測度在53±1.2 mV/pH 之間,
drift 也小於0.1mV/hr。另外我們在EGFET 上固定尿素酶,並使用尿素溶液來測量,此實驗
可以證明EGFET 上附著有硝化纖維素後仍具有固定蛋白質的能力,本裝置在尿素溶液濃度
為64 mg/dl 中測試反應電壓為l54 mV。硝化纖維素表面的多孔性證明具有少的障壁,在20
秒內可快速反應完畢。作過穩定測試後,我們發現此感測器在量測尿素溶液時,元件重複
使用至第27 次後仍然維持有非常好的特性。
Nitrocellulose membrane is a high quality membrane for immobilizing protein and nucleic acid
which in suitable for applications such as western southern, northern transfer, rapid
immunochromatographic devices and protein microarrays. In this paper, we first propose a
nitrocellulose modified extended-gate field effect transistors (EGFET) . The nitrocellulose film
was deposited on SnO2/ITO gate directly by spin-coated technology. The optimized
nitrocellulose film manufacturing conditions were discussed through by factors of spin speed of
the spin coater concentration of nitrocellulose and distilled water and examine under which the
devices were examined by performing the pH sensitive experiments to understand the
characteristics of sensitivity. The result shows that the manufacturing factors of 3% pure water,
9% nitrocellulose and 3000 rpm spin rate can form an optimize EGFET characteristic of 53±1.2
mV/pH sensitivity and <0.1mV/hr drift. Additional, we immobilized the urease to measure urea
to understand the protein immobilization ability of nitrocellulose modified EGFET. The device
shows a response of 154mV in 64mg/dL concentration of urea. The porous surface of
nitrocellulose shows low diffusion barrier, which has a fast response time of 20 secs. After a
stability test, we found the sensor could maintain its excellent performance for detection of urea
samples at least 27 times. In contrast to the immobilization by entrapment method, this
nitrocellulose shows similar immobilization rate, but lower diffusion barrier.
Appears in Collections:[生物科技系暨生物醫學研究所] 研究計劃

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