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Title: Aspergillus terreus 固定在絲瓜上進行重金屬吸附之最適化探討
Study on immobilization and metal biosorption of Aspergillus terreus on loofah sponge
Authors: 田汶玄
Wen-Xuan Tian
Contributors: 生物科技研究所
Keywords: 生物吸附
heavy metal
Date: 2006
Issue Date: 2008-12-25 09:54:39 (UTC+8)
Abstract: 本研究主題在於比較顆粒與磨碎的固定化菌體(FBILS)與游離菌體(FFB)對重金屬的吸附能力,並尋找其最佳吸附條件。研究結果顯示,固定化菌體的對重金屬吸附能力優於游離的菌體,而吸附能力的順序為鉛>汞>鎘。另外最佳操作條件探討,我們也發現隨著金屬溶液中初始濃度的增加,固定化及游離菌體對金屬的吸附能力也會增加。此外,固定化及游離菌體吸附金屬離子的最佳pH值為5~6,且溫度對固定化菌體吸附金屬離子並沒有顯著的影響。實驗結果顯示,Langmuir等溫吸附模式較Freundlich等溫吸附模式更能描述這株菌體吸附金屬離子之行為。本研究也探討複合金屬對固定化及游離菌體競爭吸附能力之影響,其順序為鉛>汞>鎘。實驗最後以0.05 N HNO3(aq)對磨碎的固定化菌體做再生試驗,結果顯示其脫附效率可以高達99%,且經過5次的循環回收,仍具有70 %以上的吸附能力。總結固定化菌體除具有較佳的物理化學性質,且可應用於脫除廢水中之鉛、汞、鎘,是一相當有潛力的水質淨化方法。
The aim of this study is to compare the adsorption efficiency of immobilized fungal biomass attached on loofah sponge ( including ungrounded and grounded FBILS) with free fungal biomass (FFB) to treat heavy metal solution. Results showed that FBILS was more effective than FFB to adsorb heavy metal from aqueous solution. Metals were selectively removed by FBILS and FFB systems, with the removal efficiency of Pb(II) >Hg(II) >Cd(II). The maximum uptake of metal ions was obtained at pH 5~6. Temperature had no obvious effect on the biosorption ability of FBILS. The Langmuir model was better than the Freundlich model to describe the sorption phenomena of the three metals to FBILS and FFB. Study results showed the competitive adsorption ability in the order of Pb(II)> Hg(II) >Cd(II). The desorption efficiency was as high as 99%, where the regenerated grounded FBILS was suitable to be for five cycles, of which still maintain 70% adsorption ability. Our results have demonstrated FBILS, with higher chemical and physical stability, can be readily applied to treat industrial wastewater.
Appears in Collections:[醫學檢驗生物技術系碩士班] 博碩士論文

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