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Please use this identifier to cite or link to this item: http://ir.hwai.edu.tw:8080/ir/handle/310996100Q/1699

Title: 甲殼素應用在化妝品抑菌劑之研究
The Study of Chitosan Used for Bacteriostatin in Cosmetics
Authors: 張秋筠
Jhang, Ciouyun
Keywords: 幾丁聚醣
Chitosan
Date: 2012
Issue Date: 2013-12-18 09:18:59 (UTC+8)
Abstract: 本研究之目的主要是探討幾丁聚醣在化妝品中之抑菌能力,分別以不同濃度、不同分子量及pH值測定其抑菌效果,篩選出最佳抑菌濃度及pH值,並進行細胞存活率試驗、細胞毒性試驗、重金屬檢驗及微生物檢驗等試驗。
實驗選用樣品為幾丁聚醣,主要是因其溶解度較幾丁質高。幾丁聚醣不具毒性且具有良好的生物相容性與生物可分解性,對環境友善的高分子材料,在本篇研究中,我們選用三種不同分子量的幾丁聚醣,將幾丁聚醣(分子量100,000~200,000 datons)分別配製成0.5% ( pH 3.2和pH 5.6 )、1% ( pH 3.3和pH 5.7 )及1.5% ( pH 5.7 ),幾丁聚醣(分子量100,000~300,000 datons)濃度配製成0.5% ( pH 5.7 )、1% ( pH 5.7 )及2% ( pH 3.4 ),幾丁聚醣(分子量200,000~400,000 datons)濃度配製成1% ( pH 5.7 ),並以乳酸為溶劑,使幾丁聚醣分子量降解,藉此改善幾丁聚醣的溶解性質後,進行抑菌試驗分析,實驗結果發現幾丁聚醣(分子量100,000~200,000 datons)在濃度為0.5%且pH值為5.6時,對大腸桿菌、金黃色葡萄球菌均具有抑菌效果,而幾丁聚醣(分子量100,000~300,000 datons)與幾丁聚醣(分子量200,000~400,000 datons)的抑菌效果較差;同時,將濃度為0.5% (pH 5.6)、1% (pH 5.7)的幾丁聚醣(島久試藥)和濃度為1% (pH 5.7)的幾丁聚醣(分子量100,000~300,000 datons)與乳酸(對照組)及化妝品中常見抑菌劑(Chlorhexidine Gluconate、Microcare MTI)進行細胞存活率(MTT)及細胞毒性(LDH)試驗,實驗數據結果發現,乳酸(對照組)及化妝品中常見抑菌劑皆具細胞毒性且細胞無存活,然而不同濃度的幾丁聚醣對細胞具有良好存活率且不具細胞毒性。
將濃度為1.5%,pH 5.7的幾丁聚醣(分子量100,000~200,000 datons)進行重金屬檢驗,實驗結果為ND,此即表示幾丁聚醣不含重金屬元素。另外,將配製濃度為1 %、pH 5.6和0.5%、pH 5.6及1.5 %、pH 5.7的幾丁聚醣進行微生物檢測,結果顯示本研究中之幾丁聚醣之綠膿桿菌、大腸桿菌、金黃色葡萄球菌及總生菌數的檢測值為ND ,此即表示本研究之樣品不含上述之菌種(表示未檢出)。
Purpose of this study was to investigate chitosan in cosmetics in the antimicrobial activity, respectively, at different concentrations and different molecular weight and pH were measured inhibitory effect, filter out the best inhibitory concentration and pH, and the cell survival rate test, cytotoxicity test, heavy metal testing and microbiological testing and other tests.
Experimental use of sample to chitosan is mainly because of its better solubility than chitin. Chitosan non-toxic and biocompatible with Biodegradable, environmentally friendly polymer materials. In this study, we used three different molecular weight chitosan, the chitosan (MW:100,000~200,000 datons) were prepared as 0.5% (pH 3.2 and pH 5.6)、1% (pH3.3 and pH5.7) and 1.5% (pH5.7) chitosan (MW:100,000~300,000 datons) concentration of the preparation of 0.5% (pH 5.7), 1% (pH 5.7) and 2% (pH3.4) chitosan (MW:200,000~400,000 datons) concentration of the preparation into 1% (pH 5.7), and lactic acid as the solvent, the degradation of chitosan molecular weight, to improve the dissolution properties of chitosan the antibacterial agents antibacterial test analysis, experimental data and found that chitosan (MW:100,000~200,000 datons) at a concentration of 0.5% and the pH value of 5.6, that for Escherichia coli, Staphylococcus aureus all have antibacterial effects, the chitosan and chitosan (MW:200,000~400,000 datons) the inhibitory effect of poor; the same time, the concentration of 0.5%(pH5.6), 1%(pH5.7) chitosan (MW:100,000~200,000 datons) and a concentration of 1% (pH 5.7) of the chitin polymer sugar and lactic acid (control group) and a common antibacterial agent of cosmetics for cell survival and cell toxicity test, experimental results showed that lactic acid (control group) and common cosmetics and antibacterial agent with a cytotoxic cell-free survival, and different concentrations of chitosan on cell survival (MTT) and do not have good cytotoxicity (LDH).
The concentration of 1.5%, pH 5.7 chitosan (MW:100,000~200,000 datons) for heavy metals testing, test data results for the ND, namely, that chitosan does not contain heavy metals. In addition, the preparation of a concentration of 1%, pH 5.6 and 0.5%, pH 5.6 and 1.5%, pH 5.7 chitosan for microbiological testing, results showed that chitosan on Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and total plate count detection is ND, namely, that the study sample dose not contain the above strains (that is not detected).
Appears in Collections:[生物科技系暨生物醫學研究所] 博碩士論文

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